| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220351 |
| Chromosome: | chromosome 6 |
| Location: | 3836942 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278171 | CGL132 | predicted protein; (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTGCGCCTGGTGCATACCAGCGCCCGT |
| Internal bar code: | GGATCGGGGACTGGCCTCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 99.8694 |
| LEAP-Seq n confirming: | 2294 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACACCCGAAGGTGTAGCC |
| Suggested primer 2: | CTACAGCTGCAACAGGAGCA |