Insertion junction: LMJ.RY0402.220363_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.220363
Chromosome:chromosome 9
Location:1758843
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396451 GOX15 (1 of 1) IPR011043//IPR014756//IPR015202 - Galactose oxidase/kelch, beta-propeller // Immunoglobulin E-set // Domain of unknown function DUF1929; Glyoxal oxidase 15 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACGCCGCCGGCCCGCACTGCGCGCGCTG
Internal bar code:TTCTGACTCCACGCCGTTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:796
LEAP-Seq percent confirming:99.2348
LEAP-Seq n confirming:2853
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGCTTGCCACCCACTTAC
Suggested primer 2:ACAACCCAAATTCCAATCCA