Insertion junction: LMJ.RY0402.220367_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.220367
Chromosome:chromosome 9
Location:4031095
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g393062 (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTACACACGCACACACACACACTCACCGC
Internal bar code:GGAGGAACGCCAAGGCACGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:848
LEAP-Seq percent confirming:72.939
LEAP-Seq n confirming:1159
LEAP-Seq n nonconfirming:430
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGTACTCACCATTGTCG
Suggested primer 2:CACTCACACACTCACACCCC