| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220386 |
| Chromosome: | chromosome 2 |
| Location: | 3946627 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096551 | (1 of 230) IPR017986 - WD40-repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATGATTAGCTAAAGGGATGGTAGAGGG |
| Internal bar code: | TGTAAGGTGAAACACGAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 93.4087 |
| LEAP-Seq n confirming: | 496 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGTGTGTGTGTGATTGTG |
| Suggested primer 2: | TTGTAACCCCCTTACCCCTC |