Insertion junction: LMJ.RY0402.220392_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.220392
Chromosome:chromosome 7
Location:2326838
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g327950 MAW9 Membrane-associated hydroxyproline-rich glycoprotein 9 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATAACCAAAGGCGGCACGGCACGCACAG
Internal bar code:GGTTAGGCCCAAGACCGAGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1030
LEAP-Seq percent confirming:99.2248
LEAP-Seq n confirming:8832
LEAP-Seq n nonconfirming:69
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAGATCGACGAAGGGGTA
Suggested primer 2:AAACAGAAGGGGACATCACG