Insertion junction: LMJ.RY0402.220414_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.220414
Chromosome:chromosome 9
Location:1789683
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g396250 VTE5 Phosphatidate cytidylyltransferase; (1 of 1) K18678 - phytol kinase (VTE5) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACACACCTCGCCCCCCAGTCCGGCCTC
Internal bar code:ATCCACACCAATAGCTCAGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:986
LEAP-Seq percent confirming:99.7628
LEAP-Seq n confirming:2103
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGGCAAGTACGCTGTGTG
Suggested primer 2:ACCAGATTCCTGCCAAACAC