| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220416 |
| Chromosome: | scaffold 18 |
| Location: | 220800 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749797 | FAP281 | Flagellar Associated Coiled-Coil Protein 281 | 3'UTR|3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGCTGCGAGTCTGCCCATGTGCAGCCC |
| Internal bar code: | CGTAGTTACAGCTGGGCCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 99.1585 |
| LEAP-Seq n confirming: | 18382 |
| LEAP-Seq n nonconfirming: | 156 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTTCCGGGCACGTTTATC |
| Suggested primer 2: | CTTGATGGTTCGTGACGATG |