Insertion junction: LMJ.RY0402.220417_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.220417
Chromosome:chromosome 3
Location:2120305
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g157050 (1 of 1) IPR020966 - Aluminum-activated malate transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGGCCAGGGCTGCAGGCAATGCACGGT
Internal bar code:CCCGAGTCCCAGGGCGTCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:452
LEAP-Seq percent confirming:94.9514
LEAP-Seq n confirming:12507
LEAP-Seq n nonconfirming:665
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGTCAGGAGTTTACGGAG
Suggested primer 2:AGAAGCACAGGACGATTGCT