| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220417 |
| Chromosome: | chromosome 3 |
| Location: | 2120349 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157050 | (1 of 1) IPR020966 - Aluminum-activated malate transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCGTGCTACCACGGTACCACCCTAACT |
| Internal bar code: | TTGGTAATCACTGTTTCTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.4282 |
| LEAP-Seq n confirming: | 4695 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTCAGGAGTTTACGGAG |
| Suggested primer 2: | AGAAGCACAGGACGATTGCT |