Insertion junction: LMJ.RY0402.220430_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.220430
Chromosome:chromosome 6
Location:2354768
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g267550 BBS5 Bardet-Biedl syndrome-5 associated protein; (1 of 1) K16748 - Bardet-Biedl syndrome 5 protein (BBS5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAATACGACTGCCATGTTGCACACTCGCC
Internal bar code:ATTGTGTACTACGGAACCGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:319
LEAP-Seq percent confirming:93.5675
LEAP-Seq n confirming:2691
LEAP-Seq n nonconfirming:185
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGTGGGGAGAGACGAGAT
Suggested primer 2:AGGAAAACAAAGCGCAAAGA