| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220436 |
| Chromosome: | chromosome 9 |
| Location: | 7840763 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416700 | Related to rhamnosyl methyltransferase; (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTCATCGCCCGGACATAACCGGGGTCG |
| Internal bar code: | GAGTTCTCGCGAGAATGGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 92.5067 |
| LEAP-Seq n confirming: | 6901 |
| LEAP-Seq n nonconfirming: | 559 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAAATCTTGTGCAACAGC |
| Suggested primer 2: | CAAAGGGAGAGGATGTTGGA |