Insertion junction: LMJ.RY0402.220438_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.220438
Chromosome:chromosome 11
Location:2460292
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g475550 UMM10 (1 of 2) 2.1.1.165 - Methyl halide transferase / Methyl chloride transferase; methyl-halide synthetase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTCGCACCGCAGCGCAGACATCTCTCG
Internal bar code:TGTGACCTATAGTCGGCAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:915
LEAP-Seq percent confirming:99.583
LEAP-Seq n confirming:3821
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACCGGTGTACGATACGA
Suggested primer 2:AAGTATGGGGCTGGTGAGTG