Insertion junction: LMJ.RY0402.220439_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.220439
Chromosome:chromosome 17
Location:4041361
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g729101 PHC37 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Putative pherophorin-chlamydomonas homolog CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCGGGCCTACCTGCCCAACCCCATCGGC
Internal bar code:ATGGTGGATGCCAGAAGTCAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:401
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTCCTTCACCTCCTCCT
Suggested primer 2:GCAGCACTTGTTGTTGCTGT