| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220453 |
| Chromosome: | chromosome 17 |
| Location: | 3428589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724450 | RAA10 | component psaA trans-splicing complex I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTTTGCATGCAGCAGTTAGCGCCATAG |
| Internal bar code: | GCTTTCCCAGCACGTCCCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 98.2021 |
| LEAP-Seq n confirming: | 2021 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTGTGGATTGAGCATCG |
| Suggested primer 2: | TGCTGAATGCAAGGACAGAC |