| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220474 |
| Chromosome: | chromosome 12 |
| Location: | 4766744 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524000 | SUC1,GTR7 | (1 of 1) 2.4.1.14 - Sucrose-phosphate synthase / UDP-glucose-fructose-phosphate glucosyltransferase; Sucrose-phosphate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGGCGCAGAGTTGAGAGAGGCATTGTC |
| Internal bar code: | CCAAATAGTGCTTTTCGACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 93.3476 |
| LEAP-Seq n confirming: | 435 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGGGTCGCAAGTAGGAG |
| Suggested primer 2: | CGATTAGAGGAACAGCCGAG |