Insertion junction: LMJ.RY0402.220483_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.220483
Chromosome:chromosome 4
Location:2251811
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g220150 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGCTGAACATGAACCTCGCTCAACACA
Internal bar code:CTCCTATCTCTACGGTTGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:415
LEAP-Seq percent confirming:99.2272
LEAP-Seq n confirming:4879
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCTTGAGGGTTCAGGGTC
Suggested primer 2:CTTGACTGCTCCACCTCCTC