| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220511 |
| Chromosome: | chromosome_12 |
| Location: | 6955253 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g560350 | CNK2 | NimA-related protein kinase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCATAAACACGCATACCGTAAAACCCGCAC |
| Internal bar code: | GTCGCCCTGTGGGGTGGAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 97.3117 |
| LEAP-Seq n confirming: | 6009 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTCTGTGGTGTCCCAAG |
| Suggested primer 2: | ACAACAGCAACAACAGCAGC |