| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220512 |
| Chromosome: | chromosome 1 |
| Location: | 3006505 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018550 | CYG69 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGACTCAAACCATCCGCTCATTGTTGT |
| Internal bar code: | GGTTAGGGTGACTCTCGAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 99.4412 |
| LEAP-Seq n confirming: | 5339 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGATCAGCGACATCTT |
| Suggested primer 2: | GAGTGAGGAGTGAGGGCTTG |