| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220519 |
| Chromosome: | chromosome 9 |
| Location: | 1645716 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397095 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGATGTGCGATGGGACAGTGGCAAGGC |
| Internal bar code: | GCCAATCGTCGTAGTGGCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 98.3478 |
| LEAP-Seq n confirming: | 5774 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTCGTGGGCTGTAAGGTG |
| Suggested primer 2: | GCATACGTCTGTTTGAGCGA |