| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220522 |
| Chromosome: | chromosome 14 |
| Location: | 2641872 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626200 | P4H7,PFH7,PHX18 | Prolyl 4-hydroxylase 7; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGAGGCGGAGTCGAGGAGATGCACTGG |
| Internal bar code: | CCTGGCCTCAGGTACGTAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 98.0769 |
| LEAP-Seq n confirming: | 357 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCGGATGTTACAGAGGG |
| Suggested primer 2: | CCACCTTCCATTCCATGAAC |