| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220525 |
| Chromosome: | chromosome 12 |
| Location: | 4085510 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517800 | (1 of 18) PF12906 - RING-variant domain (RINGv) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACGATCCTCTCATAAGCCCCTGCCAGT |
| Internal bar code: | TTGCGTAAGATCGAAGCTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 99.5872 |
| LEAP-Seq n confirming: | 2171 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGCAGCTGACAGCATA |
| Suggested primer 2: | TGTTCAACACACCCGGTCTA |