| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220528 |
| Chromosome: | chromosome_1 |
| Location: | 31264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g000100 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCCATATCCGGGCGCACGGCGGGGCCATC |
| Internal bar code: | TTGTTGCGTAAAAAGAGTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 96.4137 |
| LEAP-Seq n confirming: | 11103 |
| LEAP-Seq n nonconfirming: | 413 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTGCAAACCAGGGGTAG |
| Suggested primer 2: | AGTTGCTGATGCAACACTGC |