| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220528 |
| Chromosome: | chromosome 17 |
| Location: | 6007686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740700 | FAP336 | (1 of 7) PF05517 - p25-alpha (p25-alpha); P25 Alpha Domain Containing Flagellar Associated Protein 336 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGTGGCTGACTCCCTCTTTCCCATAC |
| Internal bar code: | AAGCTGGACGCTTGTGTGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1187 |
| LEAP-Seq percent confirming: | 98.4853 |
| LEAP-Seq n confirming: | 832745 |
| LEAP-Seq n nonconfirming: | 12808 |
| LEAP-Seq n unique pos: | 503 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCTCAAGGGCCTCTCTT |
| Suggested primer 2: | TTTGTGGGTGTGTGTGTGTG |