| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220538 |
| Chromosome: | chromosome 7 |
| Location: | 2816634 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331800 | CPK1,CGL79 | conserved protein with carbohydrate kinase motif; (1 of 1) PTHR10584//PTHR10584:SF153 - SUGAR KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGTCGTCTCAGCTGCCGCCTACGCGA |
| Internal bar code: | GCAGGATTAACGGCGCAAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 99.2099 |
| LEAP-Seq n confirming: | 40054 |
| LEAP-Seq n nonconfirming: | 319 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGGAGACAGCAGGAAG |
| Suggested primer 2: | GAGTACCGAGCCAAGCTGTC |