Insertion junction: LMJ.RY0402.220545_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.220545
Chromosome:chromosome 17
Location:5945814
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g740261 FAP365 (1 of 96) IPR019734 - Tetratricopeptide repeat; Flagellar Associated Protein 365 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGGTGTTGCCCCCGCGCCGGAACACAGC
Internal bar code:CAAATCGGATTCGAGCTGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1095
LEAP-Seq percent confirming:99.6837
LEAP-Seq n confirming:10715
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATTGCGATAGGGCTTTG
Suggested primer 2:GTACCCGTTACTGTTGCCGT