| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220545 |
| Chromosome: | chromosome_17 |
| Location: | 5945814 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g740261 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACGGTGTTGCCCCCGCGCCGGAACACAGC |
| Internal bar code: | CAAATCGGATTCGAGCTGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1095 |
| LEAP-Seq percent confirming: | 99.6837 |
| LEAP-Seq n confirming: | 10715 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATTGCGATAGGGCTTTG |
| Suggested primer 2: | GTACCCGTTACTGTTGCCGT |