Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.220555 |
Chromosome: | chromosome_13 |
Location: | 124471 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g562800 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCCTAACGCCTCGGCCTGTTCTCCATTAG |
Internal bar code: | CCCCAGGCTTAGGCCATACGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 97 |
LEAP-Seq percent confirming: | 93.7966 |
LEAP-Seq n confirming: | 2389 |
LEAP-Seq n nonconfirming: | 158 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGTGTGTGTTACCTCCCCC |
Suggested primer 2: | CCTCCCCAACTCTCACACAC |