| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220567 |
| Chromosome: | chromosome 3 |
| Location: | 5943492 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190250 | SUB6 | (1 of 5) PTHR10795//PTHR10795:SF157 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // MEMBRANE-BOUND TRANSCRIPTION FACTOR PEPTIDASE, SITE 1; Subtilisin-like protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCTCCCGCGCAACTTCCAGCTCCCAGC |
| Internal bar code: | GTTTCGCCCCCAACGGATGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 79.8578 |
| LEAP-Seq n confirming: | 337 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCGAGTTGAAGTGCAT |
| Suggested primer 2: | GCCAGGTAGAGGCAGCATAG |