| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220589 |
| Chromosome: | chromosome 14 |
| Location: | 51925 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g608150 | (1 of 1) PTHR32387:SF0 - NO VEIN-LIKE PROTEIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTCCGAGTCAGCGCCGCGCCGCAGGTG |
| Internal bar code: | TGCCCGACTTTAATATCCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 955 |
| LEAP-Seq percent confirming: | 63.9224 |
| LEAP-Seq n confirming: | 6095 |
| LEAP-Seq n nonconfirming: | 3440 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCGTATCTTCCTTGATA |
| Suggested primer 2: | AGTGCCAACCACAGAGCAG |