Insertion junction: LMJ.RY0402.220593_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.220593
Chromosome:chromosome 6
Location:2435921
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g268550 GTR,CGL121,CSL1 Cellulose-synthase-like 1; (1 of 1) 2.4.1.32 - Glucomannan 4-beta-mannosyltransferase / Glucomannan-synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACAGTGCTCCATCCACGCACACTACTGA
Internal bar code:CGAGGGAGGATCGTGCTAATGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:201
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGATTGTTCATGCCACG
Suggested primer 2:CGCTGCTTACTTCATCACCA