| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220626 |
| Chromosome: | chromosome 2 |
| Location: | 2257755 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090150 | CAF2,PANC,PAN3,PANC1 | (1 of 1) 6.3.2.1 - Pantoate--beta-alanine ligase (AMP-forming) / Pantothenate synthetase; Pantothenate synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGCGCCCATGTACAAGAACGCTGCAA |
| Internal bar code: | GAGGGGATGCTGCCTGGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 96.8424 |
| LEAP-Seq n confirming: | 9017 |
| LEAP-Seq n nonconfirming: | 294 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGTGTTGAAGAGGGGG |
| Suggested primer 2: | GACCAAATCGGATGATGACC |