| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220634 |
| Chromosome: | chromosome 3 |
| Location: | 5148046 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182050 | LCS1 | (1 of 4) K01897 - long-chain acyl-CoA synthetase (ACSL, fadD); Long-chain acyl-CoA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGTCGGGTTGATGCAGCTTCCGCTCT |
| Internal bar code: | GTGCTCCTCCATAGTTCCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 98.3193 |
| LEAP-Seq n confirming: | 234 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGATTGCAAGTGCCTGG |
| Suggested primer 2: | TTAGCTGCAGGATGTCAACG |