| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220638 |
| Chromosome: | chromosome 12 |
| Location: | 6499113 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538550 | (1 of 21) IPR000679 - Zinc finger, GATA-type | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACACAAAGCCTGTAGTACACATTTAAG |
| Internal bar code: | CCCCTATTCTGAACGGGCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1145 |
| LEAP-Seq percent confirming: | 98.9045 |
| LEAP-Seq n confirming: | 15258 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACCGTTTCCGCTTTGTG |
| Suggested primer 2: | AAGGTATGGCAGGACATTCG |