| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220640 |
| Chromosome: | chromosome 6 |
| Location: | 1351879 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258700 | PYC1 | (1 of 1) 6.4.1.1 - Pyruvate carboxylase / Pyruvic carboxylase; Pyruvate carboxylase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGACACCTCAGTCCTTACTGGTACAAC |
| Internal bar code: | GCCCCTTCATGAAGGGAGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 99.0484 |
| LEAP-Seq n confirming: | 2394 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTCTTGAAAGGGTCTTG |
| Suggested primer 2: | GTGCAGAGAAGGTTGCATGA |