| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220654 |
| Chromosome: | chromosome 3 |
| Location: | 8302771 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199150 | (1 of 1) PTHR12126//PTHR12126:SF1 - NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED // SUBFAMILY NOT NAMED; NAD dependent epimerase/dehydratase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCCGCGCCCCGCAGGTGCTCTGTGTA |
| Internal bar code: | GTCCTGACAGAGCCCCAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.8476 |
| LEAP-Seq n confirming: | 655 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGAGGCATGTTGGCACTG |
| Suggested primer 2: | CTGTTGGCATAACATGTCCG |