| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220660 |
| Chromosome: | chromosome 6 |
| Location: | 5032511 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280350 | (1 of 5) IPR001680//IPR011047//IPR017986//IPR020472 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain // G-protein beta WD-40 repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTAGCGGCTCCTTCTGGCCCTGGCTCAG |
| Internal bar code: | CGGTGAATTCACATGTTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 99.5546 |
| LEAP-Seq n confirming: | 6482 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTCGACCGTGGTCTATT |
| Suggested primer 2: | CTTGCCCTGGTAGTCCCATA |