Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.220684 |
Chromosome: | chromosome_2 |
Location: | 611737 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g077850 | FAP212 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGTATTCATGCTGAACGCTATTCGACTA |
Internal bar code: | TACTCTTGGGTCCGGGGGTCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 908 |
LEAP-Seq percent confirming: | 99.4995 |
LEAP-Seq n confirming: | 18688 |
LEAP-Seq n nonconfirming: | 94 |
LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTGCAAACACCCATTTTT |
Suggested primer 2: | GGGACCGAAGCTTTTACCTC |