| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220684 |
| Chromosome: | chromosome_2 |
| Location: | 611738 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077850 | FAP212 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGGCTCCTGGGCTCGAGCCCAGGGCCTAT |
| Internal bar code: | GGTACGAGAAGCTTTGATGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 95.5107 |
| LEAP-Seq n confirming: | 14063 |
| LEAP-Seq n nonconfirming: | 661 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCAAACACCCATTTTT |
| Suggested primer 2: | GGGACCGAAGCTTTTACCTC |