| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220715 |
| Chromosome: | chromosome 17 |
| Location: | 1928387 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710600 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGTGGGGGCGGGGGTTACAGCCGGGGC |
| Internal bar code: | TACTTTTAGGTCTACTGTTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 99.3991 |
| LEAP-Seq n confirming: | 5128 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGAGGGTGGAGAACTG |
| Suggested primer 2: | CATCCGCGTTGTTATTGTTG |