| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220717 |
| Chromosome: | chromosome 1 |
| Location: | 6979032 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050400 | TIM22C | Mitochondrial inner membrane translocase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTCGCTCGCAGCCCAGCCGCCCAGAGG |
| Internal bar code: | TTGATTGGGCCGGCCGTGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 97.446 |
| LEAP-Seq n confirming: | 35331 |
| LEAP-Seq n nonconfirming: | 926 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGGGTCTTTGACTTTGG |
| Suggested primer 2: | CATGGCGTTAGAGAGCACAA |