Insertion junction: LMJ.RY0402.220725_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.220725
Chromosome:chromosome 3
Location:3755851
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g169900 (1 of 4) IPR023222 - PsbQ-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCAGTCCCCGCAAACCGCAACGCTTG
Internal bar code:AGTTACGGATATTTATAGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:99.0701
LEAP-Seq n confirming:5327
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:GACAGGGAGGTGAAGGATGA
Suggested primer 2:GAGATTGGGAATGACGAGGA