Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.220725 |
Chromosome: | chromosome_3 |
Location: | 3755851 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g169900 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCACCAGTCCCCGCAAACCGCAACGCTTG |
Internal bar code: | AGTTACGGATATTTATAGGACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 947 |
LEAP-Seq percent confirming: | 99.0701 |
LEAP-Seq n confirming: | 5327 |
LEAP-Seq n nonconfirming: | 50 |
LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACAGGGAGGTGAAGGATGA |
Suggested primer 2: | GAGATTGGGAATGACGAGGA |