| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220738 |
| Chromosome: | chromosome 13 |
| Location: | 2107738 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577650 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGCTGAGGAGGTGTGGGGTCACGCAT |
| Internal bar code: | TGCGTCCAACGATCGGAGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 99.577 |
| LEAP-Seq n confirming: | 23775 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTATGGTTGCTGTTTG |
| Suggested primer 2: | AGCAGGGATTTGGGCTAGAT |