| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220759 |
| Chromosome: | chromosome 10 |
| Location: | 5987175 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462650 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCTCTTGGGCCTTGGGGGATGCCGGCA |
| Internal bar code: | AACACACGTCTTAATCGCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 99.4571 |
| LEAP-Seq n confirming: | 8976 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATCGTCTCCAGCTCCTC |
| Suggested primer 2: | GCTGGAGGATACCTGGCATA |