| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220766 |
| Chromosome: | chromosome 6 |
| Location: | 4043718 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278208 | (1 of 1) PF04845 - PurA ssDNA and RNA-binding protein (PurA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGACCGGGACTACGAATGAACCGGTGCC |
| Internal bar code: | ATGATACCGAAGAGGACTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 793 |
| LEAP-Seq percent confirming: | 96.5625 |
| LEAP-Seq n confirming: | 5562 |
| LEAP-Seq n nonconfirming: | 198 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCAATGCCAGCAGCTAA |
| Suggested primer 2: | ACTTACGTCAAGCCAGGAGC |