| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220799 |
| Chromosome: | chromosome 1 |
| Location: | 4894286 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033950 | EXN2 | 3'-5' exonuclease with GRF zinc finger domain; (1 of 1) K18417 - ERI1 exoribonuclease 2 [EC:3.1.-.-] (ERI2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTAACCGCAGTGAAAGTCTGGGAGGCAC |
| Internal bar code: | ACGCTCACATGGCTCCTTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.3532 |
| LEAP-Seq n confirming: | 34256 |
| LEAP-Seq n nonconfirming: | 223 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTGTGTCATAGTCCTCG |
| Suggested primer 2: | GTTTTTGAACGTGTTGGGCT |