| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220811 |
| Chromosome: | chromosome 1 |
| Location: | 4310752 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029150 | DAU1,DNAAF1,LRRC50,ODA7,ODA7A | (1 of 2) PTHR24365:SF315 - DYNEIN ASSEMBLY FACTOR 1, AXONEMAL; Outer row dynein assembly protein 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTGCGGCTGCGCTCCTCCTCCTCCTC |
| Internal bar code: | AACGGCCAGTGGGGTATAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1081 |
| LEAP-Seq percent confirming: | 99.8061 |
| LEAP-Seq n confirming: | 3603 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGACTGCAAGTAAATGGC |
| Suggested primer 2: | CCTGAAGTGCCTGTGGGTAT |