| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220818 |
| Chromosome: | chromosome 3 |
| Location: | 4138116 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173450 | PAO4 | Pheophorbide a oxygenase-related protein; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGCGCCGCATCACTCCTTACTTCTTG |
| Internal bar code: | GTTCGAGTCAGGGAGTGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 94 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCTTTTTCAACACACGC |
| Suggested primer 2: | TGCACTTGCAGGAAGATGAC |