Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.220818 |
Chromosome: | chromosome 6 |
Location: | 7947906 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g303500 | (1 of 1) K13101 - G patch domain and KOW motifs-containing protein (GPKOW) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCAACTCCATTGACCCCCGTACTGCAC |
Internal bar code: | GGTTTCAAGGGACAGGTGAATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1023 |
LEAP-Seq percent confirming: | 99.7366 |
LEAP-Seq n confirming: | 1893 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCGTTAGCCTCTGTTGAAG |
Suggested primer 2: | ACAAGGACAGGGAACGTGAC |