| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220820 |
| Chromosome: | chromosome 11 |
| Location: | 1742869 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467789 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAATCGCCAGGAAACTGATACGCTAGG |
| Internal bar code: | GAGGAATTCCATGCCAGGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 98.1748 |
| LEAP-Seq n confirming: | 2528 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATTACCCTCATCCAGGC |
| Suggested primer 2: | AAAGTTTGTATGTTGCGCCC |