Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.220826 |
Chromosome: | chromosome 1 |
Location: | 2426410 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g013500 | (1 of 6) PF01814 - Hemerythrin HHE cation binding domain (Hemerythrin) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAATCCGCATCCATACAAGTCGTGTCA |
Internal bar code: | ATCAGACCCTCCTCCTAGAGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 583 |
LEAP-Seq percent confirming: | 88.7324 |
LEAP-Seq n confirming: | 189 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACATAAGGCAACGCTTGGAC |
Suggested primer 2: | TAGACGGGGGAGGAGTTCTT |