| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220843 |
| Chromosome: | chromosome 12 |
| Location: | 3658914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514250 | HLM17 | (1 of 1) PF08429 - PLU-1-like protein (PLU-1); Histone-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCTCCGCCTCGTCCTCCACGCCATCCA |
| Internal bar code: | ACGGGGGCATAGTCGTTAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 99.883 |
| LEAP-Seq n confirming: | 5978 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCAGAGACCAACACCAC |
| Suggested primer 2: | ATCCTGCTGTGTGACTGCTG |